Human Vimentin antibody

Vimentin is a type III intermediate filament (IF) protein that is expressed in mesenchymal cells. IF proteins are found in all metazoan cells as well as bacteria. IF, along with tubulin-based microtubules and actin-based microfilaments, comprises the cytoskeleton. All IF proteins are expressed in a highly developmentally-regulated fashion; vimentin is the major cytoskeletal component of mesenchymal cells. Because of this, vimentin is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastaticprogression.

Product Specifications

Product Name Alternative

Vimentin, epididymis luminal protein 113, HEL113, CTRCT30

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human VIM (1-466aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT5H8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Vimentin, Vimentin, epididymis luminal protein 113, HEL113, CTRCT30, ATGA0453-10 µg, ATGA0453-20 µg, ATGA0453-50 µg, ATGA0453-100 µg, ATGA0453-250 µg, ATGA0453-500 µg, ATGA0453-1 mg, ATGA0453-10, ATGA0453-20, ATGA0453-50, ATGA0453-100, ATGA0453-250, ATGA0453-500, ATGA0453-1

References & Citations

John E. Eriksson., et al. (2009) J Clin Invest. 119 (7) : 1763-1771. ; ; Cabeen MT., et al. (2010) Annu Rev Genet. 44: 365-392.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_003371

Uniprot Accession Number

P08670

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Jurkat cell lysate ; ; Lane 2 : K562 cell lysate ; ; Lane 3 : 293T cell lysate ; ; Lane 4 : A549 cell lysate ; ; Lane 5 : SK-OV-3 cell lysate ; ; Lane 6 : HeLa cell lysate

IF Description

ICC/IF analysis of VIM in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human VIM antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0453-IF1b.jpg}