Human GMF-beta/GMFB antibody

GMFB, also known as GMF, belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMFB in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMFB expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF.

Product Specifications

Product Name Alternative

Glia maturation factor beta, GMF, GMF beta, Glia maturation factor beta

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GMFB (1-142aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT44D8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GMF-b, GMFB, Glia maturation factor beta, GMF, GMF beta, Glia maturation factor beta, ATGA0452-10 µg, ATGA0452-20 µg, ATGA0452-50 µg, ATGA0452-100 µg, ATGA0452-250 µg, ATGA0452-500 µg, ATGA0452-1 mg, ATGA0452-10, ATGA0452-20, ATGA0452-50, ATGA0452-100, ATGA0452-250, ATGA0452-500, ATGA0452-1

References & Citations

Li YL., et al. (2010) Eur J Cancer. 46 (11) : 2104-18. ; ; Wada T., et al. (2009) Int J Oncol. 35 (5) : 973-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_004115

Uniprot Accession Number

P60983

WB Description

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: PC3 cell lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GMFB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: GMFB transfected 293T cell lysate {ATGA0452-WB2.jpg}

IF Description

ICC/IF analysis of GMFB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GMFB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .