Human Thioredoxin Reductase 1/TRXR1 antibody

TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotidedisulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases.

Product Specifications

Product Name Alternative

GRIM 12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, TR, TR 1, TRXR 1, TXNR, TXNRD 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human TXNRD1 (161-647aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT30F1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GRIM 12, GRIM12, GRIM-12, KDRF, KM 102 derived reductase like factor, MGC9145, Oxidoreductase, Thioredoxin reductase, Thioredoxin reductase 1, Thioredoxin reductase 1 cytoplasmic, Thioredoxin reductase 1 Gene associated with retinoid IFN induced mortality 12 protein, Thioredoxin reductase GRIM 12, Thioredoxin reductase GRIM12, TR, TR 1, TR1, TR-1, TRXR 1, TRXR1, TRXR-1, TXNR, TXNRD 1, TXNRD1, TXNRD-1, ATGA0451-10 µg, ATGA0451-20 µg, ATGA0451-50 µg, ATGA0451-100 µg, ATGA0451-250 µg, ATGA0451-500 µg, ATGA0451-1 mg, ATGA0451-010, ATGA0451-020, ATGA0451-050, ATGA0451-100, ATGA0451-250, ATGA0451-500, ATGA0451-01M

References & Citations

Ma X., et al. (2002) J Biol Chem. 277 (25) : 22460-8. ; ; Javvadi P (2010) Cancer Res. 70 (5) : 1941-50.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001087240

Uniprot Accession Number

Q16881

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TXNRD1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: PC3 cell lysate

IF Description

ICC/IF analysis of TXNRD1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0451-IF1b.jpg}ICC/IF analysis of TXNRD1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXNRD1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0451-IF2a.jpg} {ATGA0451-IF2b.jpg}