Human Aldehyde dehydrogenase 2/ALDH2 antibody

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations.

Product Specifications

Product Name Alternative

Aldehyde dehydrogenase 2 family member, Aldehyde dehydrogenase mitochondrial, ALDH class 2, ALDH-E2, ALDHI, ALDM

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ALDH2 (18-517aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT2C10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ALDH2, Aldehyde dehydrogenase mitochondrial, ALDH-E2, ALDHI, ALDM, Aldehyde dehydrogenase, Mitochondrial Acetaldehyde dehydrogenase 2, Aldehyde dehydrogenase 2 family, Aldehyde dehydrogenase 2 family (mitochondrial), ALDHE2, ALDH 2, ALDH class 2, ALDH E2, Liver mitochondrial ALDH, MGC1806, Mitochondrial aldehyde dehydrogenase 2, Nucleus encoded mitochondrial aldehyde dehydrogenase 2, ATGA0450-10 µg, ATGA0450-20 µg, ATGA0450-50 µg, ATGA0450-100 µg, ATGA0450-250 µg, ATGA0450-500 µg, ATGA0450-1 mg, ATGA0450-010, ATGA0450-020, ATGA0450-050, ATGA0450-100, ATGA0450-250, ATGA0450-500, ATGA0450-01M

References & Citations

Crabb DW., et al. (1989) J Clin Invest. 83 (1) : 314-6. ; ; Feng Liu., et al. (2002) Plant Physiol. 130 (4) : 1657-1674.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Redox Proteins

NCBI Accession Number

NP_000681

Uniprot Accession Number

P05091

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HepG2 cell lysate ; ; Lane 2. : Mouse liver tissue lysate ; ; Lane 3. : Mouse lung tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : ALDH2 transfected 293T cell lysate {ATGA0450-WB2.jpg}

IF Description

ICC/IF analysis of ALDH2 in A549 cells line, stained with monoclonal anti-human ALDH2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .