Human PPME1 antibody

PPME1, also known protein phosphatase methylesterase-1 (PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile.

Product Specifications

Product Name Alternative

Protein phosphatase methylesterase 1, FLJ22226, PME-1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPME1 (1-386aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT29G3

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PPME1, Protein phosphatase methylesterase 1, FLJ22226, PME-1, ATGA0449-10 µg, ATGA0449-20 µg, ATGA0449-50 µg, ATGA0449-100 µg, ATGA0449-250 µg, ATGA0449-500 µg, ATGA0449-1 mg, ATGA0449-10, ATGA0449-20, ATGA0449-50, ATGA0449-100, ATGA0449-250, ATGA0449-500, ATGA0449-1

References & Citations

Ogris E., et al. (1999) J. Biol. Chem. 274: 14382-14391.; ; Gagnon S.N., et al. (2002) Biochem. J. 368: 263-271.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_057231

Uniprot Accession Number

Q9Y570

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPME1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: A549 cell lysate