Human AK1 antibody

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and erythrocytes. It is a small ubiquitous enzyme which is essential for maintenance and cell growth. Defects in AK1 are the cause of a form of hemolytic anemia.

Product Specifications

Product Name Alternative

Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, Adenylate kinase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AK1 (1-194aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

AK1, Adenylate kinase isoenzyme1, ATP-AMP transphosphorylase 1, Myokinase, ATGA0447-10 µg, ATGA0447-20 µg, ATGA0447-50 µg, ATGA0447-100 µg, ATGA0447-250 µg, ATGA0447-500 µg, ATGA0447-1 mg, ATGA0447-10, ATGA0447-20, ATGA0447-50, ATGA0447-100, ATGA0447-250, ATGA0447-500, ATGA0447-1

References & Citations

Terzic A., et al. (2007) J Biol Chem. 282 (43) : 31366-72.; ; Morelli A., et al. (2007) Curr Eye Res. 32 (3) : 249-57.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000467

Uniprot Accession Number

P00568

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: U87-MG cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: 293T cell lysate ; ; Lane 5.: HepG2 cell lysate ; ; Lane 6.: A549 cell lysate ; ; Lane 7.: MCF7cell lysate ; ; Lane 8.: SK-OV-3 cell lysate ; ; Lane 9.: PC3 cell lysate

IF Description

ICC/IF analysis of AK1 in HeLa cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .{ATGA0447-IF1b.jpg}ICC/IF analysis of AK1 in Hep3B cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .{ATGA0447-IF2a.jpg}{ATGA0447-IF2b.jpg}ICC/IF analysis of AK1 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human AK1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0447-IF3a.jpg}{ATGA0447-IF3b.jpg}