Human AST1/GOT1 antibody

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Product Specifications

Product Name Alternative

Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Aspartate transaminase 1, AST1, SGOT, AST, cAspAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase, Cytoplasmic, cCAT, Transaminase A

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GOT1 (1-413aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT3G11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GOT1, Glutamate oxaloacetate transaminase 1 soluble, cAspAT, ATGA0445-10 µg, ATGA0445-20 µg, ATGA0445-50 µg, ATGA0445-100 µg, ATGA0445-250 µg, ATGA0445-500 µg, ATGA0445-1 mg, ATGA0445-10, ATGA0445-20, ATGA0445-50, ATGA0445-100, ATGA0445-250, ATGA0445-500, ATGA0445-1

References & Citations

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22) : 5293-9. ; ; Totan A., et al. (2006) Clin Chem Lab Med. 44 (5) : 612-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002070

Uniprot Accession Number

P17174

WB Description

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : A427 cell lysate The recombinat proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant human GOT1 ; ; Lane 2 : Recombinant human GOT2 {ATGA0445-WB2.jpg} The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : 293T cell lysate ; ; Lane 2 : GOT1 Transfected 293T cell lysate {ATGA0445-WB3.jpg}

FACS Description

Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0445-FC2.jpg}

IF Description

ICC/IF analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0445-IF2.jpg}