Human NCS1 antibody

NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. It is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Specifications

Product Name Alternative

Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NCS1 (1-190aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT11B8

Conjugation

Unconjugated

Applications

ELISA, WB, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NCS1, Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1., ATGA0442-10 µg, ATGA0442-20 µg, ATGA0442-50 µg, ATGA0442-100 µg, ATGA0442-250 µg, ATGA0442-500 µg, ATGA0442-1 mg, ATGA0442-10, ATGA0442-20, ATGA0442-50, ATGA0442-100, ATGA0442-250, ATGA0442-500, ATGA0442-1

References & Citations

Nakamura TY., et al (2001) Proc. Natl. Acad. Sci. u.S.A. 98: 12808-12813. ; ; Bahi N., et al (2003) Hum. Mol. Genet. 12: 1415-1425.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_055101

Uniprot Accession Number

P62166

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: LnCap cell lysate ; ; Lane 4.: HeLa cell lysate

FACS Description

Flow cytometry analysis of NCS1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of NCS1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {2004010381410490.jpg}