Human beta 2-Microglobulin/B2M antibody

Beta2 microglobulin, also known as B2M, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Increased production or destruction of these cells causes B2M levels in the blood to increase. This increase is seen in people with cancers involving white blood cells, but it is particularly meaningful in people newly diagnosed with multiple myeloma. Multiple myeloma is a malignancy (cancer) of a certain kind of white blood cell, called a plasma cell. B2M Testing is done primarily when evaluating a person for certain kinds of cancer affecting white blood cells including chronic lymphocytic leukemia, non-Hodgkin's lymphoma, and multiple myeloma or kidney disease.

Product Specifications

Product Name Alternative

Beta-2-microglobulin, CDABP0092, HDCMA22P

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human B2M (21-119aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT101F10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

B2-Microglobulin, Beta-2-microglobulin, CDABP0092, HDCMA22P, ATGA0439-10 µg, ATGA0439-20 µg, ATGA0439-50 µg, ATGA0439-100 µg, ATGA0439-250 µg, ATGA0439-500 µg, ATGA0439-1 mg, ATGA0439-10, ATGA0439-20, ATGA0439-50, ATGA0439-100, ATGA0439-250, ATGA0439-500, ATGA0439-1

References & Citations

Huang WC., et al (2010) J Biol Chem. 285 (11) : 7947-56. ; ; Morabito A., et al. (2009) Hum Immunol. 70 (7) : 492-5.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis. Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_004039

Uniprot Accession Number

P61769

WB Description

The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human B2M antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HeLa cell lysate ; ; Lane 2 : U937 cell lysate

FACS Description

Flow cytometry analysis of B2M in A431 cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of B2M in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0439-FC2.jpg}

IF Description

ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0439-IF1b.jpg}