Human BAG2 antibody

BCL2-associated athanogene 2, also known as BAG2, is a member of the Bag family of proteins. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain. BAG2 is a major component of the HSC 70/CHIP chaperone-dependent ubiquitin ligase complex and acts to disrupt CHIP-mediated ubiquitylation.

Product Specifications

Product Name Alternative

BCL2-associated athanogene 2, BAG-2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human BAG2 (1-211aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT29E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

BAG2, BCL2-associated athanogene 2, BAG-2, ATGA0438-10 µg, ATGA0438-20 µg, ATGA0438-50 µg, ATGA0438-100 µg, ATGA0438-250 µg, ATGA0438-500 µg, ATGA0438-1 mg, ATGA0438-10, ATGA0438-20, ATGA0438-50, ATGA0438-100, ATGA0438-250, ATGA0438-500, ATGA0438-1

References & Citations

Takayama S., et al. (1999) J Biol Chem. 274: 781-786.; ; Arndt V., et al. (2005) CHIP Mol Biol Cell. 16: 5891-5900.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_004273

Uniprot Accession Number

O95816

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAG2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: A549 cell lysate ; ; Lane 3.: K562 cell lysate ; ; Lane 4.: HepG2 cell lysate ; ; Lane 5.: HeLa cell lysate

IF Description

ICC/IF analysis of BAG2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BAG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0438-IF1b.jpg}