Human IMP3 antibody

IMP3 is the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the u3 snoRNP complex. The protein contains an S4 domain.

Product Specifications

Product Name Alternative

U3 small nucleolar ribonucleoprotein protein IMP3, BRMS2, C15orf12, MRPS4

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IMP3 (1-184aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT32E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IMP3, u3 small nucleolar ribonucleoprotein protein IMP3, BRMS2, C15orf12, MRPS4, ATGA0437-10 µg, ATGA0437-20 µg, ATGA0437-50 µg, ATGA0437-100 µg, ATGA0437-250 µg, ATGA0437-500 µg, ATGA0437-1 mg, ATGA0437-10, ATGA0437-20, ATGA0437-50, ATGA0437-100, ATGA0437-250, ATGA0437-500, ATGA0437-1

References & Citations

Li C., et al. (2007) Mod Pathol. 20 (2) : 242-7. ; ; Granneman S., et al. (2003) Nucleic Acids Res. 31 (7) : 1877-87.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_060755

Uniprot Accession Number

Q9NV31

WB Description

The Cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IMP3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : IMP3 Transfected 293T cell lysate

IF Description

ICC/IF analysis of IMP3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IMP3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of IMP3 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human IMP3 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0437-IF2.jpg}