Human FKBP22/FKBP14 antibody

FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.

Product Specifications

Product Name Alternative

22 kDa FK506 binding protein, FK506 binding protein 14 (22 kDa), FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human FKBP14 (20-211aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT18E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

22 kDa FK506 binding protein, FK506 binding protein 14, FK506 binding protein 14 (22 kDa), FKBP 22, FKBP22, Peptidyl prolyl cis trans isomerase, Peptidyl-prolyl cis-trans isomerase FKBP14, PPIase, Rotamase, FKBP14, FKBP 14, ATGA0436-10 µg, ATGA0436-20 µg, ATGA0436-50 µg, ATGA0436-100 µg, ATGA0436-250 µg, ATGA0436-500 µg, ATGA0436-1 mg, ATGA0436-010, ATGA0436-020, ATGA0436-050, ATGA0436-100, ATGA0436-250, ATGA0436-500, ATGA0436-01M

References & Citations

Tremmel D., et al. (2007) J Mol Biol. 369 (1) : 55-68. ; ; Budiman C., et al. (2009) FEBS J. 276 (15) : 4091-101.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Development

NCBI Accession Number

NP_060416

Uniprot Accession Number

Q9NWM8

WB Description

The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate The cell lysates (20 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FKBP14 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : FKBP14 Transfected 293T cell lysate {ATGA0436-WB2.jpg}

IF Description

ICC/IF analysis of FKBP14 in HeLa cells. The cell was stained with ATGA0436 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .