Human Calbindin 2/CALB2 antibody

CALB2 is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers.

Product Specifications

Product Name Alternative

CR, Calretinin, CALB2, CAL2, CAB29, 29 kDa calbindin

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CALB2 (1-271aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT5C5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Calretinin, ATGA0435-10 µg, ATGA0435-20 µg, ATGA0435-50 µg, ATGA0435-100 µg, ATGA0435-250 µg, ATGA0435-500 µg, ATGA0435-1 mg, ATGA0435-10, ATGA0435-20, ATGA0435-50, ATGA0435-100, ATGA0435-250, ATGA0435-500, ATGA0435-1

References & Citations

Rogers JH., et al. (1987) J Cell Biol. 105 (3) : 1343-1353.; ; Dreher B., et al. (1996) J. Comp. Neurol. 376 (2) : 223-40.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

AAH15484

Uniprot Accession Number

P22676

WB Description

The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CALB2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate

IF Description

ICC/IF analysis of CALB2 in HeLa cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .