Human Inorganic Pyrophosphatase/PPA1 antibody

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation.

Product Specifications

Product Name Alternative

Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PPA1 (1-289aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b λ

Clone

AT4G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Inorganic Pyrophosphatase, PPA1, Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061, ATGA0433-10 µg, ATGA0433-20 µg, ATGA0433-50 µg, ATGA0433-100 µg, ATGA0433-250 µg, ATGA0433-500 µg, ATGA0433-1 mg, ATGA0433-10, ATGA0433-20, ATGA0433-50, ATGA0433-100, ATGA0433-250, ATGA0433-500, ATGA0433-1

References & Citations

Carman GM, et al., (2006) Trends Biochem. Sci. 31 (12) : 694-9. ; ; Usui Y, et al., (2010) J. Dent. Res. 89 (5) : 504-9.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_066952

Uniprot Accession Number

Q15181

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : A431 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4. : HepG2 cell lysate ; ; Lane 5. : U87MG cell lysate ; ; Lane 6. : PC3 cell lysate ; ; Lane 7. : WiDr cell lysate

FACS Description

Flow cytometry analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .

IF Description

ICC/IF analysis of Pyrophosphatase/PPA1 in HeLa cells. The cell was stained with ATGA0433 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .