Human Cystatin A antibody

CSTA, also known as Stefin A, is thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. The protein is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. CSTA is thought to be a tumor suppressor, since an inverse correlation seems to exist between the level of CSTA and tumor progression, in several malignancies.

Product Specifications

Product Name Alternative

Cystatin-A, STF1, STFA

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CSTA (1-98aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT1B12

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Cystatin A, Cystatin-A, STF1, STFA, ATGA0432-10 µg, ATGA0432-20 µg, ATGA0432-50 µg, ATGA0432-100 µg, ATGA0432-250 µg, ATGA0432-500 µg, ATGA0432-1 mg, ATGA0432-10, ATGA0432-20, ATGA0432-50, ATGA0432-100, ATGA0432-250, ATGA0432-500, ATGA0432-1

References & Citations

Takai T., et al. (2009) J Immuno. 183 (12) : 7958-65.; ; Sinha AA., et al. (2008) Anticancer Res. 28 (4B) : 2271-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_005204

Uniprot Accession Number

P01040

WB Description

The A431 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CSTA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of CSTA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CSTA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .