Human DECR1 antibody

DECR1 is a mitochondrial protein that exists as a homotetramer and belongs to the family of short-chain dehydrogenases/reductases. It functions as an auxiliary enzyme of beta-oxidation andt participates in the metabolism of unsaturated fatty enoyl-CoA esters. Specifically, DECR1 uses NADP+ to catalyze the reduction of 2, 4-dienoyl-CoA to yield trans-3-enoyl-CoA, which can then be used as an intermediate in the Krebs cycle. Additionally, DECR1 is thought to function as a tumor suppressor, possibly downregulating the expression of Neu and slowing the rate of tumorigenesis.

Product Specifications

Product Name Alternative

24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human DECR1 (35-335aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT3B2

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

DECR1, 24-dienoyl-CoA reductase mitochondrial, 2,4-dienoyl-CoA reductase, mitochondrial, DECR, NADPH, SDR18C1, ATGA0431-10 µg, ATGA0431-20 µg, ATGA0431-50 µg, ATGA0431-100 µg, ATGA0431-250 µg, ATGA0431-500 µg, ATGA0431-1 mg, ATGA0431-10, ATGA0431-20, ATGA0431-50, ATGA0431-100, ATGA0431-250, ATGA0431-500, ATGA0431-1

References & Citations

Koivuranta KT., et al. (1994) Biochem J. 304 (3) : 787-92.; ; Roe CR., et al. (1990) J Clin Invest. 85 (5) : 1703-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001350

Uniprot Accession Number

Q16698

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DECR1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: PC3 cell lysate ; ; Lane 2.: HepG2 cell lysate

FACS Description

Flow cytometry analysis of DECR1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of DECR1 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DECR1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .