Human CDC37 antibody

The protein encoded by this gene is highly similar to CDC37, a cell division cycle control protein of Saccharomyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK, as well as eIF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

Product Specifications

Product Name Alternative

Cell division cycle 37 homolog, P50CDC37, CDC37, Cell division cycle 37 homolog, Cell division cycle 37 homolog S cerevisiae hypothetical protein CDC37, CDC 37, CDC37 cell division cycle 37 homolog, Cdc37 homolog, CDC37 protein, Hsp90 chaperone protein kinase targeting subunit, Hsp90 chaperone protein kinase targeting subunit p50Cdc37, Hsp90 co chaperone Cdc37, p50, p50Cdc37.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CDC37 (1-378aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT3G7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CDC37, Cell division cycle 37 homolog, P50CDC37, CDC37, Cell division cycle 37 homolog, Cell division cycle 37 homolog S cerevisiae hypothetical protein CDC37, CDC 37, CDC37 cell division cycle 37 homolog, Cdc37 homolog, CDC37 protein, Hsp90 chaperone protein kinase targeting subunit, Hsp90 chaperone protein kinase targeting subunit p50Cdc37, Hsp90 co chaperone Cdc37, p50, p50Cdc37., ATGA0428-10 µg, ATGA0428-20 µg, ATGA0428-50 µg, ATGA0428-100 µg, ATGA0428-250 µg, ATGA0428-500 µg, ATGA0428-1 mg, ATGA0428-10, ATGA0428-20, ATGA0428-50, ATGA0428-100, ATGA0428-250, ATGA0428-500, ATGA0428-1

References & Citations

Quotti Tubi, et al. (2013) J Hematol Oncol. 6: 78.; ; Manni S., et al. (2012) Clin Cancer Res. 18: 1888-900.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_008996

Uniprot Accession Number

Q16543

WB Description

The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDC37 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Jurkat cell lysate ; ; Lane 2. : A431 cell lysate ; ; Lane 3. : K562 cell lysate ; ; Lane 4. : LnCap cell lysate ; ; Lane 5. : SW480 cell lysate ; ; Lane 6. : HeLa cell lysate ; ; Lane 7. : MCF7 cell lysate ; ; Lane 8. : 293T cell lysate

IF Description

ICC/IF analysis of CDC37 in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDC37 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .