Human Thioredoxin-2 antibody

Thioredoxin (Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis.

Product Specifications

Product Name Alternative

MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Thioredoxin2 (60-166aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1B1

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Thioredoxin2, MTRX, TRX2, MT-TRX, Thioredoxin2, mitochondrial thioredoxin, thioredoxin 2 precursor, Thioredoxin mitochondrial, TRX 2, TXN 2, TXN2, Thioredoxin-2, ATGA0427-10 µg, ATGA0427-20 µg, ATGA0427-50 µg, ATGA0427-100 µg, ATGA0427-250 µg, ATGA0427-500 µg, ATGA0427-1 mg, ATGA0427-10, ATGA0427-20, ATGA0427-50, ATGA0427-100, ATGA0427-250, ATGA0427-500, ATGA0427-1

References & Citations

Wang Z., et al. (2007) Rapid Commun Mass Spectrom. 21 (22) : 3658-66.; ; Zhang H., et al. (2007) Arch Biochem biophys. 465 (1) : 119-26.; ; Holmgren, A. (1979) Journal of Biological Chemistry. 254, 9627-9632.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036605

Uniprot Accession Number

Q99757

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HepG2 cell lysate ; ; Lane 2 : HeLa cell lysate ; ; Lane 3 : Raji cell lysate ; ; Lane 4 : K562 cell lysate ; ; Lane 5 : Jurkat cell lysate ; ; Lane 6 : A431 cell lysate ; ; Lane 7 : MCF7 cell lysate ; ; Lane 8 : THP1 cell lysate Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Thioredoxin2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant human Thioredoxin1 protein ; ; Lane 2 : Recombinant human Thioredoxin2 protein {ATGA0427-WB2.jpg}

IF Description

ICC/IF analysis of TXN2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TXN2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .