Human Creatine kinase B/CKB antibody

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, tumor and cancer cells.

Product Specifications

Product Name Alternative

Creatine kinase B-type, Brain creatine kinase, Creatine kinase B chain, Creatine phosphokinase B-type, CPK-B, B-CK, CKBB

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CKB (1-381aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT8E4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Creatine Kinase BB, Creatine kinase B-type, B-CK, CKBB, Creatine kinase B-type, BCK, ATGA0426-10 µg, ATGA0426-20 µg, ATGA0426-50 µg, ATGA0426-100 µg, ATGA0426-250 µg, ATGA0426-500 µg, ATGA0426-1 mg, ATGA0426-10, ATGA0426-20, ATGA0426-50, ATGA0426-100, ATGA0426-250, ATGA0426-500, ATGA0426-1

References & Citations

Chang EJ., et al. (2008) Nat Med. 14 (9) : 966-72. ; ; Debrincat MA., et al. (2007) J Biol Chem. 282 (7) : 4728-37.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001814

Uniprot Accession Number

P12277

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKB antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 2.: LnCap cell lysate

FACS Description

Flow cytometry analysis of CKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .