Human UDP-glucose dehydrogenase/UGDH antibody

UGDH is a member of the UDP-glucose/GDP-mannose dehydrogenase family and is a ubiquitously expressed protein most abundant in the liver. This protein converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis.

Product Specifications

Product Name Alternative

UDP-glucose 6-dehydrogenase, UDP-Glc dehydrogenase, UDP-GlcDH, UDPGDH

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human UGDH (1-494aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT2G11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

UGDH, uDP-glucose dehydrogenase, GDH, uDP-GlcDH, uDPGDH, uGD, uDP-glucose dehydrogenase, uDP-glucose 6-dehydrogenase, ATGA0425-10 µg, ATGA0425-20 µg, ATGA0425-50 µg, ATGA0425-100 µg, ATGA0425-250 µg, ATGA0425-500 µg, ATGA0425-1 mg, ATGA0425-10, ATGA0425-20, ATGA0425-50, ATGA0425-100, ATGA0425-250, ATGA0425-500, ATGA0425-1

References & Citations

Bontemps Y., et al. (2003) J Biol Chem. 278 (24) : 21566-75.; ; Vatsyayan J., et al. (2006) Biosci Biotechnol Biochem. 70 (2) : 401-10.; ; Egger S., et al. (2011) J Biol Chem. 286 (27) : 23877-87.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_003350

Uniprot Accession Number

O60701

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UGDH antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH-3T3 cell lysate ; ; Lane 3.: HepG2 cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: MCF7 cell lysate

IF Description

ICC/IF analysis of UGDH in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UGDH antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0425-IF1b.jpg}