Human Enolase 1/2 antibody

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma.

Product Specifications

Product Name Alternative

Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, PPH, Plasminogen-binding protein, ENO1L1, MBPB1, MPB1, ENO1-IT1, ENO1 intronic transcript 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Alpha-enolase (1-434aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1G7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Enolase 2, ENO2, P09104, NP_001966.1, 2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1, ENO1L1, ENO2, Enolase, Enolase 1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, Enolase 2, Enolase-1, MBP 1, MBP1, MBP-1, MBPB1, MPB 1, MPB1, MYC promoter binding protein 1, MYC promoter-binding protein 1, NNE, Non neural enolase, Non-Neuronal Enolase (NNE), NP_001966.1, P09104, Phosphopyruvate hydratase, Plasminogen binding protein, PPH, tau-crystallin, ATGA0424-10 µg, ATGA0424-20 µg, ATGA0424-50 µg, ATGA0424-100 µg, ATGA0424-250 µg, ATGA0424-500 µg, ATGA0424-1 mg, ATGA0424-010, ATGA0424-020, ATGA0424-050, ATGA0424-100, ATGA0424-250, ATGA0424-500, ATGA0424-01M

References & Citations

Das R., et al. (2009) Blood. 113 (22) : 5371-2. ; ; Ueno NT., et al. (2008) Cancer Res. 68 (22) : 9302-10.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001419

Uniprot Accession Number

P06733

WB Description

The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: ENO1 (Alpha-enolase) recombinant protein ; ; Lane 2.: ENO2 (Gamma-enolase) recombinant protein ; ; Lane 3.: ENO3 (Beta-enolase) recombinant protein. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ENO1, 2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: PC3 cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: HeLa cell lysate {ATGA0424-WB2.jpg}

FACS Description

Flow cytometry analysis of ENO1, 2 in LNCap cells. The cell was stained with ATGA0424 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .

IF Description

ICC/IF analysis of ENO1, 2 in HeLa cells. The cell was stained with ATGA0424 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .