Human Pontin/RUVBL1 antibody

RuVBL1, also known as TIP49A, is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This complex may be required for the activation of transcriptional programs associated with oncogene and proto oncogene mediated growth induction, tumor suppressor mediated growth arrest.

Product Specifications

Product Name Alternative

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP-54, INO80 complex subunit H, INO80H, NMP 238, Nuclear matrix protein 238, Pontin 52, RuvB like AAA ATPase 1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP49a, TIP60-associated protein 54-alpha

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human RuVBL1 (1-456aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT1D6

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

49 kDa TATA box-binding protein-interacting protein, 49 kDa TBP-interacting protein, 54 kDa erythrocyte cytosolic protein, ECP 54, ECP54, ECP-54, INO80 complex subunit H, INO80H, NMP 238, NMP238, NMP-238, Nuclear matrix protein 238, PONTIN, Pontin 52, Pontin52, Pontin-52, RuvB like AAA ATPase 1, RuVBL 1, RuVBL1, RuVBL-1, RuvB-like 1, RVB1, TAP54-alpha, TIH1, TIP 49, TIP49, TIP-49, TIP49a, TIP60-associated protein 54-alpha, ATGA0423-10 µg, ATGA0423-20 µg, ATGA0423-50 µg, ATGA0423-100 µg, ATGA0423-250 µg, ATGA0423-500 µg, ATGA0423-1 mg, ATGA0423-010, ATGA0423-020, ATGA0423-050, ATGA0423-100, ATGA0423-250, ATGA0423-500, ATGA0423-01M

References & Citations

Jha S., et al. (2009) Mol Cell. 34 (5) : 521-33.; ; Haurie V., et al. (2009) Hepatology. 50 (6) : 1871-83.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_003698

Uniprot Accession Number

Q9Y265

WB Description

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RuVBL1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Raji cell lysate

IF Description

ICC/IF analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0423-IF1b.jpg}