Human Glutathione Synthetase/GSS antibody

Glutathione synthetase, also known GSS, is the second enzyme in the glutathione biosynthesis pathway. It catalyses the condensation of gamma-glutamylcysteine and glycine, to form glutathione. Defects in GSS are the cause of glutathione synthetase deficiency (GSS deficiency) ; also known as 5-oxoprolinuria or pyroglutamic aciduria. It is a severe form characterized by an increased rate of hemolysis and defective function of the central nervous system.

Product Specifications

Product Name Alternative

Glutathione synthetase, GSHS, GSH synthetase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GSS (1-474aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT12F2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GSS, Glutathione synthetase, GSHS, GSH synthetase, ATGA0422-10 µg, ATGA0422-20 µg, ATGA0422-50 µg, ATGA0422-100 µg, ATGA0422-250 µg, ATGA0422-500 µg, ATGA0422-1 mg, ATGA0422-10, ATGA0422-20, ATGA0422-50, ATGA0422-100, ATGA0422-250, ATGA0422-500, ATGA0422-1

References & Citations

Polekhina G., et al. (1999) EMBO J. 18: 3204-3213. ; ; Huanq Z A., et al. (2000) Biochim Biophys Acta. 1493: 48-55.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000169

Uniprot Accession Number

P48637

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: MDA-MB231 cell lysate ; ; Lane 2.: WiDr cell lysate ; ; Lane 3.: SW480 cell lysate ; ; Lane 4.: A427 cell lysate ; ; Lane 5.: MCF7 cell lysate ; ; Lane 6.: 293T cell lysate ; ; Lane 7.: HeLa cell lysate ; ; Lane 8.: HT1080 cell lysate ; ; Lane 9.: U87MG cell lysate ; ; Lane 10.: A549 cell lysate

IF Description

ICC/IF analysis of GSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of GSS in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0422-IF2.jpg}