Human Jak2 antibody

JAK2 is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Product Specifications

Product Name Alternative

Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human JAK2 (1014-1132aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT53B7

Conjugation

Unconjugated

Applications

ELISA, WB, FACS, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Jak2, Janus kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK10, ATGA0421-10 µg, ATGA0421-20 µg, ATGA0421-50 µg, ATGA0421-100 µg, ATGA0421-250 µg, ATGA0421-500 µg, ATGA0421-1 mg, ATGA0421-10, ATGA0421-20, ATGA0421-50, ATGA0421-100, ATGA0421-250, ATGA0421-500, ATGA0421-1

References & Citations

Bole-Feycot C., et al. (1998) Endocr Rev. 19 (3) : 225-68. doi:10.1210/er.19.3.225.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004963

Uniprot Accession Number

O60674

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human JAK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : K-562 cell lysate ; ; Lane 2. : TF-1 cell lysate ; ; Lane 3. : 293T cell lysate ; ; Lane 4 : Jurkat cell lysate ; ; Lane 5 : THP-1 cell lysate

FACS Description

Flow cytometry analysis of JAK2 in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of JAK2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human JAK2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .