Human CapG antibody

Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments.

Product Specifications

Product Name Alternative

Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CAPG (1-348aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT1D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CapG, Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1., ATGA0420-10 µg, ATGA0420-20 µg, ATGA0420-50 µg, ATGA0420-100 µg, ATGA0420-250 µg, ATGA0420-500 µg, ATGA0420-1 mg, ATGA0420-10, ATGA0420-20, ATGA0420-50, ATGA0420-100, ATGA0420-250, ATGA0420-500, ATGA0420-1

References & Citations

Kumar A., et al. (2015) Mol Cell Endocrinol. 404: 91-101.; ; Dabiri G A., et al. (1992) J Biol Chem. 267: 16545-16552.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Immunology

NCBI Accession Number

AAH00728

Uniprot Accession Number

P40121

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH3T3 cell lysate ; ; Lane 3.: Mouse spleen tissue lysate ; ; Lane 4.: THP-1 cell lysate ; ; Lane 5.: Raw 264.7 cell lysate

IF Description

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0420-IF1b.jpg}