Human 6-phosphogluconate Dehydrogenase/PGD antibody

PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion.

Product Specifications

Product Name Alternative

6-phosphogluconate dehydrogenase, Decarboxylating, PGDH, Phosphogluconate dehydrogenase, 6PGD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PGD (1-483aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT46B3

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

6-phosphogluconate dehydrogenase, PGD, Phosphogluconate dehydrogenase, 6PGD, Phosphogluconate dehydrogenase 0610042A05Rik, Au019875, C78335, 6 phosphogluconate dehydrogenase, decarboxylating, OTTMuSP00000011754, RP23-249G3.4, ATGA0419-10 µg, ATGA0419-20 µg, ATGA0419-50 µg, ATGA0419-100 µg, ATGA0419-250 µg, ATGA0419-500 µg, ATGA0419-1 mg, ATGA0419-10, ATGA0419-20, ATGA0419-50, ATGA0419-100, ATGA0419-250, ATGA0419-500, ATGA0419-1

References & Citations

Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107 (7) : 3198-203.; ; Tagen M., et al. (2009) J Immunol. 183 (10) : 6313-9;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_002622

Uniprot Accession Number

P52209

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGD antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: MCF7 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: Jurkat cell lysate

IF Description

ICC/IF analysis of 6-phosphogluconate dehydrogenase/PGD1 in Hep3B cells. The cell was stained with ATGA0419 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .