Human Podoplanin/PDPN antibody

Podoplanin (PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor.

Product Specifications

Product Name Alternative

TIA2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, Lung type I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein isoform a AGGRuS, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, GP40, Gp38, GP36, Glycoprotein 36 KD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDPN (99-207aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT56F7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

TIA2, TIA 2, T1A-2, T1A, T1 ALPHA GENE, T1 alpha, Podoplanin, PDPN, PA2.26 antigen, PA2.26, OTTHuMP00000044504, OTTHuMP00000009640, OTS8, OTS 8, Lung type-I cell membrane-associated glycoprotein isoform a AGGRuS, Lung type-I cell membrane-associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein T1A 2, Lung type I cell membrane associated glycoprotein isoform a, Lung type I cell membrane associated glycoprotein, hT1alpha2, hT1alpha1, HT1A-1, HT1A1, HT1A 1, GP40, Gp38, GP36, GP 40, GP 38, gp 36, Glycoprotein 36 KD, Glycoprotein 36, ATGA0418-10 µg, ATGA0418-20 µg, ATGA0418-50 µg, ATGA0418-100 µg, ATGA0418-250 µg, ATGA0418-500 µg, ATGA0418-1 mg, ATGA0418-010, ATGA0418-020, ATGA0418-050, ATGA0418-100, ATGA0418-250, ATGA0418-500, ATGA0418-01M

References & Citations

Wicki A., et al. (2006) Br J Cancer. 96 (1) : 1-5.; ; Zimmer G., et al. (1999) Biochem J. 341, 277-84.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cardiovascular

NCBI Accession Number

NP_006465

Uniprot Accession Number

Q86YL7

WB Description

The HeLa cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDPN antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

IF Description

ICC/IF analysis of PDPN in Hep3B cells. The cell was stained with ATGA0418 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .