Human p27/Kip1 antibody

The cyclin-dependent kinase inhibitor p27Kip1 plays an important role in cell cycle regulation. The cyclin-dependent kinase-inhibitory activity of p27Kip1 is regulated by changes in its concentration and its subcellular localization. Cyclin-dependent kinase inhibitor p27 (kip1) is a putative tumor suppressor for human cancer.

Product Specifications

Product Name Alternative

Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CDKN1b (1-198aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7B9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

P27, Kip1, Cyclin-dependent kinase inhibitor 1B, CDKN4, KIP1, MEN1B, MEN4, P27KIP1, ATGA0417-10 µg, ATGA0417-20 µg, ATGA0417-50 µg, ATGA0417-100 µg, ATGA0417-250 µg, ATGA0417-500 µg, ATGA0417-1 mg, ATGA0417-10, ATGA0417-20, ATGA0417-50, ATGA0417-100, ATGA0417-250, ATGA0417-500, ATGA0417-1

References & Citations

Fujita N., et al. (2003) J Biol Chem. 278 (49) : 49254-60.; ; Viglietto G., et al. (2002) Nat Med. 8 (10) : 1136-44.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Cell Cycle

NCBI Accession Number

NP_004055

Uniprot Accession Number

P46527

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CDKN1b antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : MCF-7 cell lysate ; ; Lane 2 : NIH/3T3 cell lysate

IF Description

ICC/IF analysis of CDKN1b in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CDKN1b antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0417-IF1b.jpg}