Human GPD1L antibody

GPD1L is converts sn-glycerol 3-phosphate to glycerone phosphate. This protein is found in the cytoplasm, associated with the plasma membrane, where it binds the sodium channel, voltage-gated, type V, alpha subunit (SCN5A) . GPD1L is thought to affect trafficking of the cardiac sodium current to the cell surface. Mutations in GPD1L are the cause of sudden infant death syndrome (SIDS) and Brugada syndrome type 2, an autosomal dominant tachyarrhythmia.

Product Specifications

Product Name Alternative

Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GPD1L (1-351aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT14E2

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GPD1L, Glycerol-3-phosphate dehydrogenase 1-like protein, GPD1-L, ATGA0415-10 µg, ATGA0415-20 µg, ATGA0415-50 µg, ATGA0415-100 µg, ATGA0415-250 µg, ATGA0415-500 µg, ATGA0415-1 mg, ATGA0415-10, ATGA0415-20, ATGA0415-50, ATGA0415-100, ATGA0415-250, ATGA0415-500, ATGA0415-1

References & Citations

Valdivia CR., et al. (2009) Am. J. Physiol. 297: H1446-H1452.; ; Van Norstrand DW., et al. (2007) Circulation. 116: 2253-2259.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_055956

Uniprot Accession Number

Q8N335

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : MCF7 cell lysate ; ; Lane 2. : 293T cell lysate ; ; Lane 3. : Jurkat cell lysate ; ; Lane 4 : SW480 cell lysate ; ; Lane 5 : PC3 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GPD1L antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : 293T cell lysate ; ; Lane 2. : GPD1L Transfected 293T cell lysate {ATGA0415-WB2.jpg}

IF Description

ICC/IF analysis of GPD1L in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GPD1L antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .