Human CPOX antibody

Coproporphyrinogen-III oxidase, mitochondrial precursor, also known as CPOX, is a 454 amino acid mitochondrial enzyme that is localized to the inner membrane space of erythrocytes. It participates in the sixth step of heme biosynthesis by catalyzing the formation of protoporphyrinogen IX from coproporphyrinogen III. Mutations in human CPOX gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.

Product Specifications

Product Name Alternative

Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human CPOX (111-454aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT36B10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

CPOX, Coproporphyrinogen-III oxidase mitochondrial precursor, Coproporphyrinogen-III oxidase, mitochondrial precursor, CPO, CPX, HCP, ATGA0414-10 µg, ATGA0414-20 µg, ATGA0414-50 µg, ATGA0414-100 µg, ATGA0414-250 µg, ATGA0414-500 µg, ATGA0414-1 mg, ATGA0414-10, ATGA0414-20, ATGA0414-50, ATGA0414-100, ATGA0414-250, ATGA0414-500, ATGA0414-1

References & Citations

Kim DH., et al. (2013) J Biochem. 154 (6) : 551-9.; ; Barbaro M., et al. (2012) Clin Genet. 81 (3) : 249-56.; ; Rosipal R., et al. (1999) Hum Mutat. 13 (1) : 44-53.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000088

Uniprot Accession Number

P36551

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CPOX antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Recombinant protein CPOX ; ; Lane 2.: 293T cell lysate ; ; Lane 3.: HepG2 cell lysate ; ; Lane 4.: A549 cell lysate ; ; Lane 5.: Jurkat cell lysate ; ; Lane 6.: K562 cell lysate ; ; Lane 7.: LnCaP cell lysate

IF Description

ICC/IF analysis of CPOX in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CPOX antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .