Human SHMT1 antibody

Serine hydroxymethyltransferase 1, also known as SHMT1, belongs to the SHMT family. This protein is cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5 10-methylene tetrahydrofolate. Also, SHMT1 preferentially supplies one-carbon units for thymidylate biosynthesis, depletes methylenetetrahydrofolate pools for S-adenosylmethionine (SAM) synthesis by synthesizing serine, sequesters 5-methyltetrahydrofolate and inhibits.

Product Specifications

Product Name Alternative

Serine hydroxymethyltransferase 1, CSHMT, SHMT

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SHMT1 (1-483aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT26E5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SHMT1, ATGA0413-10 µg, ATGA0413-20 µg, ATGA0413-50 µg, ATGA0413-100 µg, ATGA0413-250 µg, ATGA0413-500 µg, ATGA0413-1 mg, ATGA0413-10, ATGA0413-20, ATGA0413-50, ATGA0413-100, ATGA0413-250, ATGA0413-500, ATGA0413-1

References & Citations

Garrow TA., et al. (1993) J Biol Chem. 268 (16) : 11910-6.; ; Perry C., et al. (2005) J Biol Chem. 280 (1) : 396-400. ; ; Pinthong C., et al. (2014) FEBS J. 281 (11) : 2570-83.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

AAH07979

Uniprot Accession Number

P34896

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SHMT1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: A549 cell lysate ; ; Lane 2.: HeLa cell lysate; ; Lane 3.: NIH-3T3 cell lysate ; ; Lane 4.: LnCaP cell lysate

IF Description

ICC/IF analysis of SHMT1 in Hep3B cells. The cell was stained with ATGA0413 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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