Human QPRT antibody

Quinolinate phosphoribosyltransferase, also known as QPRT, is a major enzyme in the catabolism of quinolinate. It is an intermediate in the tryptophannicotinamide adenine dinucleotide (NAD) pathway, leading to the production of nicotinic acid, carbon dioxide and pyrophosphate. Elevation of QPRT levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.

Product Specifications

Product Name Alternative

Nicotinate nucleotide pyrophosphorylase (carboxylating), Nicotinate nucleotide pyrophosphorylase carboxylating, Quinolinate phosphoribosyltransferase, QPRTase, QAPRTase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human QPRT (1-297aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT24C4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

QPRT, ATGA0412-10 µg, ATGA0412-20 µg, ATGA0412-50 µg, ATGA0412-100 µg, ATGA0412-250 µg, ATGA0412-500 µg, ATGA0412-1 mg, ATGA0412-10, ATGA0412-20, ATGA0412-50, ATGA0412-100, ATGA0412-250, ATGA0412-500, ATGA0412-1

References & Citations

Cao H., et al. (2002) Biochemistry. 41 (10) : 3520-8.; ; Liu H., et al. (2007) J Mol Biol. 373 (3) : 755-63.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_055113

Uniprot Accession Number

Q15274

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human QPRT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HepG2 cell lysate; ; Lane 2 : K562 cell lysate

IF Description

ICC/IF analysis of QPRT in Hep3B cells. The cell was stained with ATGA0412 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .