Human AIF/AIFM1 antibody

Apoptosis-inducing factor 1, also known as AIFM1, is a mitochondrial protein that translocates to the nucleus upon induction of apoptosis. AIFM1 has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIFM1 from mitochondria, but not to block its apoptogenic activity. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy.

Product Specifications

Product Name Alternative

Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, AIF, CMTX4, COWCK, COXPD6, PDCD8

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AIFM1 (98-609aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT22E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

AIF, AIFM1, Apoptosis-inducing factor 1, mitochondrial isoform 2 precursor, Apoptosis-inducing factor 1 mitochondrial isoform 2 precursor, CMTX4, COWCK, COXPD6, PDCD8, ATGA0411-10 µg, ATGA0411-20 µg, ATGA0411-50 µg, ATGA0411-100 µg, ATGA0411-250 µg, ATGA0411-500 µg, ATGA0411-1 mg, ATGA0411-010, ATGA0411-020, ATGA0411-050, ATGA0411-100, ATGA0411-250, ATGA0411-500, ATGA0411-01M

References & Citations

Tang Y., et al. (2013) J Proteomics. 91C: 200-209.; ; Kim TW., et al. (2013) Cell Death Dis. 4: e919.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_665811

Uniprot Accession Number

O95831

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Jurkat cell lysate; ; Lane 2. : HeLa cell lysate; ; Lane 3. : Hep3B cell lysate; ; Lane 4. : Raji cell lysate; ; Lane 5. : K562 cell lysate; ; Lane 6. : MCF7 cell lysate; ; Lane 7. : CTLL2 cell lysate The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIFM1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Mouse heart tissue lysate; ; Lane 2. : Mouse liver tissue lysate {ATGA0411-WB2.jpg}

IF Description

ICC/IF analysis of AIFM1 in Hep3B cells. The cell was stained with ATGA0411 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .