Human GRP58/PDIA3 antibody

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface.

Product Specifications

Product Name Alternative

Protein disulfide isomerase family A member 3, Glucose regulated protein 58kDa, GPR58, Protein disulfide isomerase-associated 3, 58 kDa microsomal protein, P58, ERp61, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, GRP57, PI-PLC, HsT17083

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PDIA3 (25-505aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT9E9

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ERp57, PDIA3, ATGA0410-10 µg, ATGA0410-20 µg, ATGA0410-50 µg, ATGA0410-100 µg, ATGA0410-250 µg, ATGA0410-500 µg, ATGA0410-1 mg, ATGA0410-10, ATGA0410-20, ATGA0410-50, ATGA0410-100, ATGA0410-250, ATGA0410-500, ATGA0410-1

References & Citations

Huppa JB., Ploegh HL. (1998) Cell. 92: 145-8.; ; Ellgaard L., Ruddock LW. (2005) EMBO Rep. 6: 28-32.; ; Jessop CE., et al. (2007) EMBO J. 26: 28-40.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005304

Uniprot Accession Number

P30101

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDIA3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : NIH3T3 cell lysate ; ; Lane 4. : Raw 264.7 cell lysate; ; Lane 5. : LNCaP cell lysate

IF Description

ICC/IF analysis of PDIA3 in Hep3B cells. The cell was stained with ATGA0410 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .