Human Isocitrate Dehydrogenase 1/IDH1 antibody

IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.

Product Specifications

Product Name Alternative

Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IDH1 (1-414aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT25H10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Isocitrate Dehydrogenase1, IDH1, ATGA0409-10 µg, ATGA0409-20 µg, ATGA0409-50 µg, ATGA0409-100 µg, ATGA0409-250 µg, ATGA0409-500 µg, ATGA0409-1 mg, ATGA0409-10, ATGA0409-20, ATGA0409-50, ATGA0409-100, ATGA0409-250, ATGA0409-500, ATGA0409-1

References & Citations

Bleeker FE., et al. (2009) Hum Mutat. 30 (1) : 7-11.; ; Zhao S., et al. (2009) Science. 324 (5924) : 261-5.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_005887

Uniprot Accession Number

O75874

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDH1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1 : HepG2 cell lysate; ; Lane 2 : A549 cell lysate ; ; Lane 3 : HeLa cell lysate; ; Lane 4 : NIH3T3 cell lysate; ; Lane 5 : Jurkat cell lysate; ; Lane 6 : K562 cell lysate; ; Lane 7 : 293T cell lysate; ; Lane 8 : MCF7 cell lysate; ; Lane 9 : LnCaP cell lysate

IF Description

ICC/IF analysis of IDH1 in Hep3B cells. The cell was stained with ATGA0409 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .