Human Isocitrate Dehydrogenase 1/IDH1 antibody
IDH1, also known as isocitrate dehydrogenase (IDHC) cytoplasmic enzyme, belongs to the isocitrate and isopropylmalate dehydrogenases family. This protein catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming alpha-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of alpha-ketoglutarate. IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.
Product Specifications
Product Name Alternative
Isocitrate dehydrogenase [NADP] cytoplasmic, IDCD, IDH, IDP, IDPC, PICD
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human IDH1 (1-414aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT25H10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
References & Citations
Bleeker FE., et al. (2009) Hum Mutat. 30 (1) : 7-11.; ; Zhao S., et al. (2009) Science. 324 (5924) : 261-5.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
Scientific Category
Metabolism
NCBI Accession Number
NP_005887
Uniprot Accession Number
O75874
WB Description
IF Description
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