Human SGTA antibody

SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protein is believed to function as a component of the androgen receptor (AR) -chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances.

Product Specifications

Product Name Alternative

Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human SGTA (1-313aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT19E8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

SGTA, ATGA0408-10 µg, ATGA0408-20 µg, ATGA0408-50 µg, ATGA0408-100 µg, ATGA0408-250 µg, ATGA0408-500 µg, ATGA0408-1 mg, ATGA0408-10, ATGA0408-20, ATGA0408-50, ATGA0408-100, ATGA0408-250, ATGA0408-500, ATGA0408-1

References & Citations

Andreyeva A., et al. (2010) PLoS One. 5: e12018.; ; Cziepluch C., et al. (1998) J Virol. 72 (5) : 4149-56.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_003012

Uniprot Accession Number

O43765

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGTA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate ; ; Lane 3.: A549 cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: LNCaP cell lysate; ; Lane 6.: 293T cell lysate

IF Description

ICC/IF analysis of SGTA in Hep3B cells. The cell was stained with ATGA0408 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .