Human Peroxiredoxin 3/PRDX3 antibody

Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration.

Product Specifications

Product Name Alternative

Thioredoxin-dependent peroxide reductase mitochondrial, Thioredoxin-dependent peroxide reductase mitochondrial, PRDX3, PRX III, Antioxidant protein 1 (AOP-1), Peroxiredoxin 3, Thioredoxin-dependent peroxide reductase, mitochondrial Antioxidant Protein 1, AOP1, MER5, PRX3, SP22, Peroxiredoxin-3

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human Peroxiredoxin3 (63-256aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG3 κ

Clone

AT1F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Peroxiredoxin3, ATGA0407-10 µg, ATGA0407-20 µg, ATGA0407-50 µg, ATGA0407-100 µg, ATGA0407-250 µg, ATGA0407-500 µg, ATGA0407-1 mg, ATGA0407-10, ATGA0407-20, ATGA0407-50, ATGA0407-100, ATGA0407-250, ATGA0407-500, ATGA0407-1

References & Citations

Tsuji K., et al. (1995) Biochem J. 307 (2) : 377-381.; ; Watabe S., et al. (1997) Eur J Biochem. 249 (1) : 52-60.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_006784

Uniprot Accession Number

P30048

WB Description

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Peroxiredoxin3 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human PRDX3 protein ; ; Lane 2.: HeLa cell lysate ; ; Lane 3.: HepG2 cell lysate ; ; Lane 4.: TF1 cell lysate ; ; Lane 5.: U87MG cell lysate ; ; Lane 6.: Raji cell lysate; ; Lane 7.: 293T cell lysate; ; Lane 8.: Jurkat cell lysate; ; Lane 9.: MCF7 cell lysate

IF Description

ICC/IF analysis of Peroxiredoxin3 in HeLa cells. The cell was stained with ATGA0407 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .