Human UDP-galactose-4-epimerase/GALE antibody

The enzyme UDP-glucose 4-epimerase, also known as UDP-galactose 4-epimerase or GALE, is a homodimeric epimerase found in bacterial, fungal, plant, and mammalian cells. This enzyme performs the final step in the Leloir pathway of galactose metabolism, catalyzing the reversible conversion of UDP-galactose to UDP-glucose. GALE tightly binds nicotinamide adenine dinucleotide (NAD+), a co-factor required for catalytic activity. Additionally, human and some bacterial GALE isoforms reversibly catalyze the formation of UDP-N-acetylgalactosamine (UDP-GalNAc) from UDP-N-acetylglucosamine (UDP-GlcNAc) in the presence of NAD+, an initial step in glycoprotein or glycolipid synthesis.

Product Specifications

Product Name Alternative

UDP-glucose 4-epimerase, Galactowaldenase, UDP-N-acetylgalactosamine 4-epimerase, UDP-GalNAc 4-epimerase, UDP-N-acetylglucosamine 4-epimerase, UDP-GlcNAc 4-epimerase, UDP-galactose 4-epimerase, short chain dehydrogenase/reductase family 1E, member 1, SDR1E1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human GALE (1-348aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT6G10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

GALE, ATGA0404-10 µg, ATGA0404-20 µg, ATGA0404-50 µg, ATGA0404-100 µg, ATGA0404-250 µg, ATGA0404-500 µg, ATGA0404-1 mg, ATGA0404-10, ATGA0404-20, ATGA0404-50, ATGA0404-100, ATGA0404-250, ATGA0404-500, ATGA0404-1

References & Citations

Holden HM., et al. (2003) J. Biol. Chem. 278 (45) : 43885-8.; ; Liu Y., et al. (1996) Biochemistry. 35 (23) : 7615-20.; ; Thoden Jb., et al. (2001) J. Biol. Chem. 276 (18) : 15131-6.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_000394

Uniprot Accession Number

Q14376

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GALE antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : MCF7 cell lysate ; ; Lane 2. : Jurkat cell lysate ; ; Lane 3. : A431 cell lysate ; ; Lane 4. : A549 cell lysate ; ; Lane 5. : HeLa cell lysate; ; Lane 6. : HepG2 cell lysate

IF Description

ICC/IF analysis of GALE in Hep3B cells. The cell was stained with ATGA0404 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .