Human VTA1 antibody

The VTA1 protein is involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. This protein is involved in the sorting and down-regulation of EGFR (By similarity) and involved in HIV-1 budding.

Product Specifications

Product Name Alternative

Vacuolar protein sorting-associated protein VTA1 homolog, DRG-1, DRG1, LIP5, SBP1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human VTA1 (1-307aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT14G10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

VTA1, ATGA0403-10 µg, ATGA0403-20 µg, ATGA0403-50 µg, ATGA0403-100 µg, ATGA0403-250 µg, ATGA0403-500 µg, ATGA0403-1 mg, ATGA0403-10, ATGA0403-20, ATGA0403-50, ATGA0403-100, ATGA0403-250, ATGA0403-500, ATGA0403-1

References & Citations

Jun Shi., et al. (2001) Brain Res. 910 (1-2) : 29-37. ; ; Fujita H., et al. (2004) J Cell Sci. 117 (Pt 14) : 2997-3009.; ; Ward DM., et al. (2005) J Biol Chem. 280 (11) : 10548-55.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Virus

NCBI Accession Number

NP_057569

Uniprot Accession Number

Q9NP79

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VTA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: 293T cell lysate; ; Lane 3.: HeLa cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: Jurkat cell lysate; ; Lane 7.: K562 cell lysate; ; Lane 8.: LnCaP cell lysate; ; Lane 9.: U937 cell lysate; ; Lane 10.: A431 cell lysate

FACS Description

Flow cytometry analysis of VTA1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of VTA1 in Hep3B cells. The cell was stained with ATGA0403 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .