Human IMPA1/IMP1 antibody

IMPA1 (inositol monophosphatase1) is responsible for the provision of inositol required for synthesis of phosphatidylinositol and polyphosphoinositides. It plays a key role in the phosphatidylinositol signaling pathway by catalyzing the hydrolysis of inositol monophosphates. This protein has been identified as the pharmacological target for lithium action in the brain.

Product Specifications

Product Name Alternative

Inositol monophosphatase 1, IMPA, IMP, IMPase 1, Inositol monophosphatase 1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human IMPA1 (1-277aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2G5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

IMPA1, IMP1, ATGA0402-10 µg, ATGA0402-20 µg, ATGA0402-50 µg, ATGA0402-100 µg, ATGA0402-250 µg, ATGA0402-500 µg, ATGA0402-1 mg, ATGA0402-10, ATGA0402-20, ATGA0402-50, ATGA0402-100, ATGA0402-250, ATGA0402-500, ATGA0402-1

References & Citations

Ganzhorn AJ., et al. (1996) Biochemistry. 35 (33) : 10957-66.; ; Parthasarathy R., et al. (1997) Brain Res. 778 (1) : 99-106.; ; Ohnishi T., et al. (2007) J Biol Chem. 282 (1) : 637-46.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_005527

Uniprot Accession Number

P29218

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IMPA1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: Jurkat cell lysate ; ; Lane 2.: Raji cell lysate ; ; Lane 3.: NIH/3T3 cell lysate

FACS Description

Flow cytometry analysis of IMPA1 in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of IMPA1 in Hep3B cells. The cell was stained with ATGA0402 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . ICC/IF analysis of IMPA1 in LNCap cells. The cell was stained with ATGA0402 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) . {2004010341156324.jpg}