Human NDRG1 antibody

N-myc downstream regulated gene (NDRG) -1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease.

Product Specifications

Product Name Alternative

N-myc downstream regulated 1, CAP43, CMT4D, DRG1, GC4, HMSNL, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated 1 TDD5, 42 kDa, anti-GC4, cmt4d, Differentiation related gene1 protein, Drg 1, hmsnl, Human mRNA for RTP complete cds, NDRG1, tdds, N myc downstream regulated gene 1, N myc downstream regulated gene 1 protein, Ndr 1, ndr1, NDRG 1, NDRG1 protein, Protein nickel specific induction protein, Nickel specific induction protein Cap43, nmsl, Nmyc downstream regulated, reduced in tumor, reducin, Nmyc downstream regulated gene1, Nmyc downstream regulated gene1 protein, Protein regulated by oxygen 1, Protein regulated by oxygen1, proxy1, Reducing agents and tunicamycin responsive protein, tunicamycin-responsive protein

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NDRG1 (1-394aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT11G4

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

NDRG1, ATGA0401-10 µg, ATGA0401-20 µg, ATGA0401-50 µg, ATGA0401-100 µg, ATGA0401-250 µg, ATGA0401-500 µg, ATGA0401-1 mg, ATGA0401-10, ATGA0401-20, ATGA0401-50, ATGA0401-100, ATGA0401-250, ATGA0401-500, ATGA0401-1

References & Citations

Van Belzen N., et al. (1997) Lab Invest. 77 (1) : 85-92.; ; Kokame K., et al. (1997) J Biol Chem. 271 (47) : 29659-29665. ; ; Zhang J., et al. (2008) Gene. 417 (1-2) : 5-12.; ; Kachhap SK., et al. (2007) PLoS ONE. 2 (9) : e844.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_006087

Uniprot Accession Number

Q92597

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NDRG1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : LnCaP cell lysate ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : PC3 cell lysate ; ; Lane 4. : Hep3B cell lysate ; ; Lane 5. : HT1080 cell lysate ; ; Lane 6. : Jurkat cell lysate ; ; Lane 7. : SW480 cell lysate

IF Description

ICC/IF analysis of NDRG1 in HeLa cells. The cell was stained with ATGA0401 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .