Human PGAM2 antibody

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Since both 3-PGA and 2-PGA are allosteric regulators of the pentose phosphate pathway (PPP) and glycine and serine synthesis pathways, respectively, PGAM2 may contribute to the biosynthesis of amino acids, 5-carbon sugar, and nucleotides precursors. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB) . Mutations in this gene cause muscle phosphoglycerate mutase deficiency, also known as glycogen storage disease X. PGAM2 is one of two PGAM subunits found in humans and is predominantly expressed in adult muscle.

Product Specifications

Product Name Alternative

Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human PGAM2 (1-253aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

AT5A7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

PGAM2, ATGA0399-10 µg, ATGA0399-20 µg, ATGA0399-50 µg, ATGA0399-100 µg, ATGA0399-250 µg, ATGA0399-500 µg, ATGA0399-1 mg, ATGA0399-10, ATGA0399-20, ATGA0399-50, ATGA0399-100, ATGA0399-250, ATGA0399-500, ATGA0399-1

References & Citations

Tsujino S., et al. (1986) The Journal of biological chemistry. 264 (26) : 15334-7.; ; Xu Y., et al. (2014) Cancer research. 74 (13) : 3630-42.; ; Tsujino S., et al. (1993) American journal of human genetics. 52 (3) : 472-7.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000281

Uniprot Accession Number

P15259

WB Description

The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombnant protein (50ng) ; ; Lane 2. : HeLa cell lysate ; ; Lane 3. : HepG2 cell lysate; ; Lane 4. : 293T cell lysate ; ; Lane 5. : Jurkat cell lysate; ; Lane 6. : NIH3T3 cell lysate; ; Lane 7. : A549 cell lysate; ; Lane 8. : MCF7 cell lysate

IF Description

ICC/IF analysis of PGAM2 in Hep3B cells. The cell was stained with ATGA0399 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .