Human ALDH5A1 antibody

This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD+-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter gamma-aminobutyric acid (GABA) . In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.

Product Specifications

Product Name Alternative

Succinate-semialdehyde dehydrogenase mitochondrial, SSADH, SSDH, Succinate-semialdehyde dehydrogenase, mitochondrial

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ALDH5A1 (48-535aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT17F5

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ALDH5A1, ATGA0398-10 µg, ATGA0398-20 µg, ATGA0398-50 µg, ATGA0398-100 µg, ATGA0398-250 µg, ATGA0398-500 µg, ATGA0398-1 mg, ATGA0398-10, ATGA0398-20, ATGA0398-50, ATGA0398-100, ATGA0398-250, ATGA0398-500, ATGA0398-1

References & Citations

Chambliss KL., et al. (1995) The Journal of Biological Chemistry. 270 (1) : 461-7.; ; Trettel F., et al. (1997) Advances in Experimental Medicine and Biology. 414: 253-60.; ; Kim YG., et al. (2009) EMBO J. 828 (7) : 959-68.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_001071

Uniprot Accession Number

P51649

WB Description

The cell lysate of HepG2 (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The tissue lysate of mouse liver (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0398-WB2.jpg}

IF Description

ICC/IF analysis of ALDH5A1 in 293T cells. The cell was stained with ATGA0398 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .