Human ALDOC antibody

ALDOC, also known as aldolase C, is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1, 6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain.

Product Specifications

Product Name Alternative

Aldolase C fructose-bisphosphate., Aldolase C, fructose-bisphosphate., ALDC

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ALDOC (1-364aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT2E11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

ALDOC, ATGA0397-10 µg, ATGA0397-20 µg, ATGA0397-50 µg, ATGA0397-100 µg, ATGA0397-250 µg, ATGA0397-500 µg, ATGA0397-1 mg, ATGA0397-10, ATGA0397-20, ATGA0397-50, ATGA0397-100, ATGA0397-250, ATGA0397-500, ATGA0397-1

References & Citations

Wang CF., et al. (2007) Anim Genet. 38 (3) : 203-210.; ; Arakaki TL., et al. (2004) Protein Sci. 13 (12) : 3077-3084.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Neuroscience

NCBI Accession Number

NP_005156

Uniprot Accession Number

P09972

WB Description

The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : PC3 cell lysate; ; Lane 2. : U87MG cell lysate ; ; Lane 3. : WiDr cell lysate ; ; Lane 4. : Jurkat cell lysate ; ; Lane 5. : Mouse brain tissue lysate

IF Description

ICC/IF analysis of ALDOC in U87MG cells. The cell was stained with ATGA0397 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ALDOC in Jurkat cells. The cell was stained with ATGA0397 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{ATGA0397-IF2.jpg}