Human EMAP-II/AIMP1 antibody

AIMP1, also known as p43, hasn't until recently been recognized for its prominent immunological functions. Together with other nonenzymatic mARS structural components AIMp2/38 and AIMp3/p18, it participates in the machinery responsible for cell-cycle control and tumor suppression.

Product Specifications

Product Name Alternative

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, EMAP2, EMAPII, p43, SCYE1

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AIMP1 (1-336aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT6D10

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

EMAP-2, AIMP1, ATGA0396-10 µg, ATGA0396-20 µg, ATGA0396-50 µg, ATGA0396-100 µg, ATGA0396-250 µg, ATGA0396-500 µg, ATGA0396-1 mg, ATGA0396-10, ATGA0396-20, ATGA0396-50, ATGA0396-100, ATGA0396-250, ATGA0396-500, ATGA0396-1

References & Citations

Liang D., et al. (2015) Int Rev Immunol. 34 (5) : 367-381.; ; Hong SH., et al. (2015) Biomaterials. 44: 45-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001135888

Uniprot Accession Number

Q12904

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AIMP1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate ; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Raji cell lysate ; ; Lane 4. : Jurkat cell lysate ; ; Lane 5. : A549 cell lysate ; ; Lane 6. : HaCaT cell lysate

FACS Description

Flow cytometry analysis of AIMP1 in Hep3B cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of AIMP1 in Hep3B cells. The cell was stained with ATGA0396 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .