What is the catalog number for Human Myc tag antibody?

The catalog number for Human Myc tag antibody is ATGA0395-100.

What sizes are available for Human Myc tag antibody?

Human Myc tag antibody is available in the following size(s): 100 µL.

Is Human Myc tag antibody in stock?

Human Myc tag antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Human Myc tag antibody

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope.

Product Specifications

Product Name Alternative

Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39, c-Myc, MRTL, MYCC

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human C-Myc (410-419aa) with tag purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT19B4

Conjugation

Unconjugated

Applications

ELISA, WB

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Myc, ATGA0395-10 µg, ATGA0395-20 µg, ATGA0395-50 µg, ATGA0395-100 µg, ATGA0395-250 µg, ATGA0395-500 µg, ATGA0395-1 mg, ATGA0395-10, ATGA0395-20, ATGA0395-50, ATGA0395-100, ATGA0395-250, ATGA0395-500, ATGA0395-1

References & Citations

Cotterman R., et al. (2008) Cancer Res. 68 (23) : 9654-9662.; ; Finver SN., et al. (1988) P NATL ACAD SCI. USA 85 (9) : 3052-3056.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

NP_002458

Uniprot Accession Number

P01106

WB Description

The recombinant human SNCA-Myc tag proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000) ; ; Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000) ; ; Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000) The recombinant proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : Recombinant Human SNCA-Myc tag (20ng) ; ; Lane 2. : Recombinant Human Myc-GST tag (50ng) ; ; Lane 3. : Recombinant Human s100A8-Myc tag (50ng) ; ; Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng) {ATGA0395-WB2.jpg}