Human AK2 antibody

Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates; this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants.

Product Specifications

Product Name Alternative

Adenylate kinase 2, Mitochondrial Adenylate kinase 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, ADK2

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human AK2 (1-239aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7E7

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Mitochondrial Adenylate kinase 2, EC2743, EC2.7.4.3, EC 2.7.4.3, D4Ertd220e, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ATP AMP transphosphorylase 2, ATP AMP transphosphorylase, ATP AMP phosphotransferase, Ak-2, AK2, Ak 2, ADK2, ADK 2, Adenylate monophosphate kinase, Adenylate kinase2, Adenylate kinase isoenzyme 2, Adenylate kinase 2 mitochondrial isoform a, Adenylate kinase 2, ATGA0393-10 µg, ATGA0393-20 µg, ATGA0393-50 µg, ATGA0393-100 µg, ATGA0393-250 µg, ATGA0393-500 µg, ATGA0393-1 mg, ATGA0393-010, ATGA0393-020, ATGA0393-050, ATGA0393-100, ATGA0393-250, ATGA0393-500, ATGA0393-01M

References & Citations

Kim D Pruitt., et al. (2012) Nucleic Acids Res. 40 (Database issue) : D130-D135.; ; Lagresle-Peyrou C., et al. (2009) Nat Genet. 41 (1) : 106-11.; ; Lee Y., et al. (1998) J. Biochem. 123 (1) : 47-54.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Apoptosis

NCBI Accession Number

NP_001616

Uniprot Accession Number

P54819

WB Description

The tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Kidney tissue lysate; ; Lane 2.: Liver tissue lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HepG2 cell lysate; ; Lane 2.: NIH/3T3 cell lysate {ATGA0393-WB2.jpg}

IF Description

ICC/IF analysis of AK2 in HeLa cells. The cell was stained with ATGA0393 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .