Human Nicotinamide N-Methyltransferase/NNMT antibody

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

Product Specifications

Product Name Alternative

Nicotinamide N-methyltransferase

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human NNMT (1-264aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

AT11G11

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Nicotinamide N-Methyltransferase, NNMT, Nicotinamide N-methyltransferase, NNMT, Nicotinamide N-methyltransferase EC 2.1.1.1 Nicotinamide N methyltransferase., ATGA0392-10 µg, ATGA0392-20 µg, ATGA0392-50 µg, ATGA0392-100 µg, ATGA0392-250 µg, ATGA0392-500 µg, ATGA0392-1 mg, ATGA0392-10, ATGA0392-20, ATGA0392-50, ATGA0392-100, ATGA0392-250, ATGA0392-500, ATGA0392-1

References & Citations

Aksoy S., et al. (1995) Genomics. 29 (3) : 555-61.; ; Gerhard DS., et al. (2004) Genome Res. 14 (10B) : 2121-7.; ; Riederer M., et al. (2009) Atherosclerosis. 204 (2) : 412-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Signal Transduction

NCBI Accession Number

NP_006160

Uniprot Accession Number

P40261

WB Description

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NNMT antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: A549 cell lysate

FACS Description

Flow cytometry analysis of NNMT in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of NNMT in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NNMT antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .