Human Arginase 2/ARG2 antibody

Arginase 2 catalyzes the same reaction as arginase 1, but differs in its tissue specificity and subcellular location. Specifically, arginase 2 localizes to the mitochondria. Arginase 2 is expressed in non-hepatic tissues, with the highest levels of expression in the kidneys, but, unlike arginase 1, is not expressed in liver.

Product Specifications

Product Name Alternative

Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase.

Host

Mouse

Antigen Species

Human

Reactivity

Human

Immunogen

Recombinant human ARG2 (23-354aa) purified from E. coli

Clonality

Monoclonal

Isotype

IgG1 κ

Clone

AT7F8

Conjugation

Unconjugated

Applications

ELISA, WB, ICC/IF, FACS

Purification Method

By protein-A affinity chromatography

Concentration

1 mg/mL (determined by BCA assay)

Additionnal Information

Arginase2, ARG2, Arginase-2 type II, Kidney arginase, Non-hepatic arginase, Arginase-2, type II Arginase II mitochondrial, Kidney type arginase, Non hepatic arginase., ATGA0389-10 µg, ATGA0389-20 µg, ATGA0389-50 µg, ATGA0389-100 µg, ATGA0389-250 µg, ATGA0389-500 µg, ATGA0389-1 mg, ATGA0389-10, ATGA0389-20, ATGA0389-50, ATGA0389-100, ATGA0389-250, ATGA0389-500, ATGA0389-1

References & Citations

Watts JA., et al. (2011) Pulm Pharmacol Ther. 24 (4) : 407-413.; ; Tadie JM., et al. (2008) Am J Physiol Lung Cell Mol Physiol. 294 (3) : 489-497.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Applications Notes

The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Scientific Category

Metabolism

NCBI Accession Number

NP_001163

Uniprot Accession Number

P78540

WB Description

The SW480 Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : SW480 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ARG2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: ARG2 recombinant protein (ATGP0591) ; ; Lane 2.: ARG1 recombinant protein (ATGP0564) {2004010328382040.jpg}

FACS Description

Flow cytometry analysis of ARG2 in SW480 cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .

IF Description

ICC/IF analysis of ARG2 in SW480 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ARG2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .